3D TISSUE CLEARING
& LIGHTSHEET IMAGING
We have developed techniques to effectively clear, label, and image whole spinal cords. We are currently developing a novel 3D imaging toolbox that will serve as a powerful tool for mapping neural circuits in the intact spinal cord, as well as for assessing plasticity and inflammation after spinal cord injury. Projects are currently available in the lab to lead new avenues of this research.
Video: 3D lightsheet image of immunostaining for neuronal soma (green) and cholinergic neurons (white).